Electrophoretic analysis of hydrolases from grown gall tissues

Running head Xyloglucan endo - hydrolases in expanding tissues

Respiratory Behavior of Bacteria-Free Crown-Gall Tissues

The respiratory processes of malignant tissues of animals and human beings have long been considered to differ in important respects from those of nonmalignant tissues of the same creatures. This difference expresses itself in several ways, especially in a low R.Q. (0.8 or less) and a decided anaerobic tendency to accumulate acid products, lactic acid in particular. It seems possible, indeed, i...

Optimization and validation of capillary electrophoretic method for the analysis of amphenicols in poultry tissues.

A simple, rapid capillary electrophoretic (CE) method for simultaneous analysis of three amphenicols (chloramphenicol, thiamphenicol and florfenicol) in poultry tissues has been developed and validated. The separation condition were: buffer solution composed with 25 mM sodium tetraborate decahydrate and 10 mM sodium dodecylsulfate, an uncoated fused-silica capillary (57 cm x 75 microm i.d.), vo...

Influence of neuraminidase treatment on the electrophoretic behaviour of angiotensin converting enzyme from human tissues.

Angiotensin converting enzyme (dipeptidyl carboxypeptidase, kininase II; EC 3.4.15.1), is a membrane bound glycoprotein, playing an important role in the renin-aldosterone system. The enzyme contains a carbohydrate moiety, consisting of fucose, mannose, galactose, N-acetylglucosamine and N-acetylneuraminic acid. Treatment with neuraminidase (EC 3.2.1.18) removes sialic acid from the molecule. T...

Electrophoretic analysis of the RNA from a mouse leukemia virus.

erythroblastosis virus was purified from culture fluids by high-speed clarification, concentration through a 5% sucrose barrier, and centrifugation through a discontinuous sucrose gradient. Viral RNA was extracted by treatment of purified virions with 0.5% sodium dodecyl sulfate or phenolisodium dodecyl sulfate. RNA profiles from virus of 24-hr culture fluids were heterodisperse and included la...